PhD student Aleksandra Medić, a STREAMLINE team member, is currently on staff exchange at the Neuroscience and Mental Health Innovation Institute, University of Cardiff, Cardiff, UK. She is learning how to generate cortical organoids from induced pluripotent stem cells derived from patients with 22q11.2 microdeletion. In the next three months, she will also be trained how to analyze the samples from 60-days-old organoids. This will allow us to better understand the pathogenesis of neurodevelopmental disorders caused by 22q11.2 microdeletion.
Cryosectioning of 60 days old organoids on cryostat, device that maintains low temperature of -20°C and cuts frozen samples of organoids in 12µm thin sections. Sections are than mounted on glass slides and are prepared for immunofluorescent staining.